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Expression (2.24) also implies that var w, = var dn (0) (2.25)

Rapid water transport across plasma membranes is facilitated by specialised water channels which transport water selectively to the exclusion of small ions and solutes. Aquaporin 1 (also called CHIP28) is a channel-forming integral glycoprotein of 28 kDa molecular mass which is expressed in erythrocytes and in cells of water-transporting tissues. In order to study the structure and organization of aquaporin 1, two-dimensional crystals were obtained for electron diffraction studies. The crystals (dimensions 1.5 2.5 m diameter) were obtained by reconstituting 2 4 mg/ml solubilized deglycosylated protein into synthetic lipid bilayers (dioleyl phosphatidyl choline) by dialysis in a lipid:protein ratio of 1 : 1 to 1 : 3 ( 7). The crystals were attached to an electron microscope grid stained with uranyl formate and dried. Electron diffraction was then performed and structure factors calculated. Five individual diffraction images containing re ections up to 12 A were aligned to a common phase origin and these phases and amplitudes were merged and used to reconstruct a projection density map at 12 A resolution of aquaporin 1 shown below.

4. When the onions are translucent, add the stew meat and brown thoroughly. 5. When meat has been browned, pour in the two bottles of Guinness beer and let the meat and onion simmer in the beer for at least a half hour to make the meat tender. While it is simmering, keep an eye on the pot to make sure it is not rapidly boiling, causing the pan to dry out. Add water if needed to keep the meat covered while it is simmering. Stir from time to time to ensure even cooking. 6. Add the two cloves of garlic sliced into slivers and continue simmering the mixture. 7. When you feel the meat is suf ciently tender, add the package of baby carrots. Continue to simmer the mixture until carrots soften. 8. When carrots have become tender, add the package of frozen peas. The peas can be kept frozen and added to the mixture while frozen. Allow the stew to simmer for about another half hour. You do not want the peas to get mushy. 9. When cooking has been completed, remove from heat and serve with some hearty bread and butter. You have just served up a wonderful meal of Mother Turbyne s Stew.

Internet protocol security (IPSec) provides encryption algorithms and a more comprehensive authentication process. It encapsulates a packet by wrapping another packet around it and then encrypts the entire packet. The encrypted stream then travels across the otherwise unsafe Internet. IPSec has been widely deployed by VPN hardware vendors and service providers, but it can be used only for systems that are IPSec-compliant. Also, all devices must use a common key for encryption, and the firewall on both sides of the VPN tunnel must be configured with similar security policies. IPSec has two encryption modes:

40 A tag is a eld inserted into a frame that provides an explicit indication of the VLAN association 41 And