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Choose a precision (the number of decimal points or the fraction denominator) from the Precision drop-down list. Choose a fraction format from the Fraction Format drop-down list. This option is available only if the format that you chose uses fractions. Horizontal places a horizontal line between stacked numerators and denominators. Diagonal uses a diagonal slash between the stacked numerator and denominator. Not Stacked uses a diagonal line between the numerator and denominator, which are not stacked. You can see the effect of each choice in the preview box.

5 Electrophoresis 5.1 Principles of Electrophoresis 5.1.1 Physical Basis 5.1.2 Historical Development of Electrophoresis 5.1.3 Gel Electrophoresis Nondenaturing Electrophoresis 5.2.1 Polyacrylamide Nondenaturing Electrophoresis 5.2.2 Protein Mass Determination by Nondenaturing Electrophoresis 5.2.3 Activity Staining 5.2.4 Zymograms Denaturing Electrophoresis 5.3.1 SDS Polyacrylamide Gel Electrophoresis 5.3.2 SDS Polyacrylamide Gel Electrophoresis in Reducing Conditions 5.3.3 Chemical Crosslinking of Proteins Quaternary Structure 5.3.4 Urea Electrophoresis Electrophoresis in DNA Sequencing 5.4.1 Sanger Dideoxynucleotide Sequencing of DNA 5.4.2 Sequencing of DNA 5.4.3 Footprinting of DNA 5.4.4 Single Strand Conformation Polymorphism Analysis of DNA Isoelectric Focusing (IEF) 5.5.1 Ampholyte Structure 5.5.2 Isoelectric Focusing 5.5.3 Titration Curve Analysis 5.5.4 Chromatofocusing Immunoelectrophoresis 5.6.1 Dot Blotting and Immunodiffusion Tests with Antibodies 5.6.2 Zone Electrophoresis/Immunodiffusion Immunoelectrophoresis 5.6.3 Rocket Immunoelectrophoresis 5.6.4 Counter Immunoelectrophoresis 5.6.5 Crossed Immunoelectrophoresis (CIE) Agarose Gel Electrophoresis of Nucleic Acids 5.7.1 Formation of an Agarose Gel 5.7.2 Equipment for Agarose Gel Electrophoresis 5.7.3 Agarose Gel Electrophoresis of DNA and RNA 5.7.4 Detection of DNA and RNA in Gels Pulsed Field Gel Electrophoresis 5.8.1 Physical Basis of Pulsed Field Gel Electrophoresis 5.8.2 Equipment Used for Pulsed Field Gel Electrophoresis 5.8.3 Applications of Pulsed Field Gel Electrophoresis Capillary Electrophoresis 5.9.1 Physical Basis of Capillary Electrophoresis 5.9.2 Equipment Used in Capillary Electrophoresis 5.9.3 Variety of Formats in Capillary Electrophoresis Electroblotting Procedures 5.10.1 Equipment Used in Electroblotting 5.10.2 Western Blotting 5.10.3 Southern Blotting of DNA 5.10.4 Northern Blotting of RNA 5.10.5 Blotting as a Preparative Procedure for Polypeptides 147 147 147 148 149 153 153 153 153 155 155 155 157 159 160 161 161 161 165 165 166 167 170 170 170 172 172 174 174 176 176 177 177 177 177 179 179 179 181 182 183 183 188 188 190 190 190 192 194 195

Belachew, M, Legrand, M, Vincent, V et al (1992) Laparoscopic placement of adjustable silicone gastric band in the treatment of morbid obesity Obesity Surgery, 5, 66 9 Belachew, M, Legrand, MJ and Vincent, V (2001) History of Lap-Band : from dream to reality Obesity Surgery, 11, 297 302 Bloomberg, RD, Fleishman, A, Nalle, JE et al (2005) Nutritional de ciencies following bariatric surgery: what have we learned Obesity Surgery, 15, 145 54 Brolin, RE, Kenler, HA, Gorman, JH and Cody, RP (1992) Long-limb gastric bypass in the superobese A prospective randomized study Annals of Surgery, 215, 387 395 Buchwald, H, Avodor, Y, Braunwald, E et al (2004) Bariatric surgery A systematic review and meta-analysis The Journal of the American Medical Association, 292, 1724 37 Buchwald, H, Schwartz, MZ and Varco, RL.

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(1973) Surgical treatment of obesity Advances in Surgery, 7, 235 55 Buchwald, H and Williams, SE (2004) Bariatric surgery worldwide 2003 Obesity Surgery 14, 1157 64 Busetto, L, Pisent, C, Rinaldi, D et al (2000) Variation in lipid levels in morbidly obese patients operated with the LAP-BAND adjustable gastric banding system: effects of different levels of weight loss Obesity Surgery, 10, 569 77 Capella, JF and Capella, RF (1999) Gastro-gastric stulas and marginal ulcers in gastric bypass procedures for weight reduction Obesity Surgery, 9, 22 7 Chevallier, J-M, Zinzindohoue, F, Douard, R et al (2004) Complications after laparoscopic adjustable gastric banding for morbid obesity: experience with 1,000 patients over 7 years Obesity Surgery, 14, 407 14 Cigaina, V, Pinato, GP, Rigo, V et al (1996) Gastric peristalsis control by in situ electrical stimulation: preliminary study.

Obesity Surgery, 6, 247 9 Clayman, RV and Williams, RD (1971) Oxalate urolithiasis following jejunoileal bypass Surgical Clinics of North America, 59, 1071 7 Cleator, IGM, Birmingham, CL, Kovacevic, S et al (2006) Long-term effect of ileogastrostomy surgery for morbid obesity on diabetes mellitus and sleep apnea Obesity Surgery, 16, 1337 41 Cowan, GSMJ, Jr, and Buf ngton, CK (1998) Signi cant changes in blood pressure, glucose and lipids with gastric bypass surgery World Journal of Surgery, 22, 987 92 DeWind, LT and Payne, JH (1976) Intestinal bypass surgery for morbid obesity: long-term results The Journal of the American Medical Association, 236, 2298 301 Deitel, M (1989) Jejunocolic and jejunoileal bypass: an historical perspective, in Surgery for the Morbidly Obese Patient (ed M Deitel), FD-Communications, Toronto, pp 81 90 (ISBN 0-8121-1136-2).